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phenylboronate polyacrylamide gel  (Bio-Rad)


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  • 97

    Structured Review

    Bio-Rad phenylboronate polyacrylamide gel
    Phenylboronate Polyacrylamide Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 10233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phenylboronate+polyacrylamide+gel/Acrylamide/10__1021_slash_jf9904315-96-22-27
    Average 97 stars, based on 10233 article reviews
    phenylboronate polyacrylamide gel - by Bioz Stars, 2026-10
    97/100 stars

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    Purification:

    Article Title: Development of a Stable Isotope Dilution Assay for an Accurate Quantification of Protein-BoundNε-(1-Deoxy-d-fructos-1-yl)-l-lysine Using a13C-Labeled Internal Standard
    Article Snippet: Syntheses of the labeled Amadori compound [C6]-N -(1-deoxy-D-fructos-1-yl)-L-lysine ([C6]-DFLys) and the labeled glycated tetrapeptide Ala-[C6]-DFLys-Leu-Gly are presented.. The compounds were used in the development of stable isotope dilution assays for the quantification of the degree of glycosylation of bovine serum albumin treated for 20 min at 95 °C in the presence of glucose.. The experiments revealed that the use of the labeled standards in combination with LC/MS allowed the exact quantification of protein-bound DFLys with the high recovery rate of 95% (at a spike level of 150 nmol/mg of protein) and a low detection limit of 5 nmol/mg of protein.

    Affinity Chromatography:

    Article Title: Development of a Stable Isotope Dilution Assay for an Accurate Quantification of Protein-BoundNε-(1-Deoxy-d-fructos-1-yl)-l-lysine Using a13C-Labeled Internal Standard
    Article Snippet: Syntheses of the labeled Amadori compound [C6]-N -(1-deoxy-D-fructos-1-yl)-L-lysine ([C6]-DFLys) and the labeled glycated tetrapeptide Ala-[C6]-DFLys-Leu-Gly are presented.. The compounds were used in the development of stable isotope dilution assays for the quantification of the degree of glycosylation of bovine serum albumin treated for 20 min at 95 °C in the presence of glucose.. The experiments revealed that the use of the labeled standards in combination with LC/MS allowed the exact quantification of protein-bound DFLys with the high recovery rate of 95% (at a spike level of 150 nmol/mg of protein) and a low detection limit of 5 nmol/mg of protein.



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    97
    Bio-Rad phenylboronate polyacrylamide gel
    Phenylboronate Polyacrylamide Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phenylboronate+polyacrylamide+gel/Acrylamide/10__1021_slash_jf9904315-96-22-27
    Average 97 stars, based on 1 article reviews
    phenylboronate polyacrylamide gel - by Bioz Stars, 2026-10
    97/100 stars
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